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Primary Antibodies



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Catalog #1: bs-6313R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Others

Conjugate: Biotin

Catalog #1: bs-6313R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Others

Conjugate: HRP

Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-4 Hydroxynonenal Polyclonal Antibody, Unconjugated (bs-6313R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Antigen: bs-6313P, 2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \\nTMB staining\\nRead the data in Microplate Reader by 450nm.
Lane 1: 4HNE-BSA (0.1 \u00b5g); Lane 2: BSA (0.1 \u00b5g) probed with 4 Hydroxynonenal Polyclonal Antibody, Unconjugated (bs-6313R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 4HNE-BSA (0.1 \u00b5g); Lane 2: BSA (0.1 \u00b5g) probed with 4 Hydroxynonenal Polyclonal Antibody, Unconjugated (bs-6313R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6313r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Others

Conjugate: Unconjugated

Antigen: bs-0362P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG (bs-0295G-HRP) at 1: 5000; \\nTMB staining; Read the data in MicroplateReader by 450nm.
Lane 1: Recombinant protein A probed with Protein A Polyclonal Antibody, Unconjugated (bs-0362R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0362R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Bacteria, Others

Conjugate: Unconjugated

Catalog #1: bs-0362R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Bacteria, Others

Conjugate: Biotin

Catalog #1: bs-0362R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Bacteria, Others

Conjugate: HRP

Catalog #1: bs-0362R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Bacteria, Others

Conjugate: FITC

Catalog #1: bs-0362R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Bacteria, Others

Conjugate: Cy3

Catalog #1: bs-0362R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Bacteria, Others

Conjugate: Cy5

Catalog #1: bs-0362R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Bacteria, Others

Conjugate: Cy7

Formalin-fixed and paraffin embedded rat skeletal muscle labeled with Anti-F-Actin Polyclonal Antibody, Unconjugated (bs-1571R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
L1 and L2 rat skeletal muscle lysates probed: : Anti-F-Actin (bs-1571R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 42kDa
A549 cell lysates probed with Anti-F-Actin Polyclonal Antibody, Unconjugated (bs-1571R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-1571R

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0004r

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0004r-biotin-100ul

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0004r-hrp-100ul

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Catalog #1: bs-0004r-cy3-100ul

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-0004r-cy5-100ul

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-0004r-cy7-100ul

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-0004r-fitc-100ul

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: FITC

Catalog #1: bs-0007R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-0007R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bs-0007R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bs-0007R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy5

Catalog #1: bs-0007R-Cy3

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy3

Catalog #1: bs-0007R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy7

Catalog #1: bs-0007R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: FITC

Catalog #1: bs-0008R-Cy3

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Cy3

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Nestin Polyclonal Antibody (bs-0008R), Unconjugated 1:300 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat hippocampus labeled with Anti-Nestin Polyclonal Antibody (bs-0008R), Unconjugated 1:300 followed by conjugation to the secondary antibody and DAB staining
Neuro-2a cells probed with Rabbit Anti-Nestin Polyclonal Antibody (bs-0008R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary Antibody.
Tissue\/cell:U-251 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Nestin) polyclonal Antibody, Unconjugated (bs-0008R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0008R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-0008R-Cy7

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Cy7

Catalog #1: bs-0008R-Cy5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Cy5

Catalog #1: bs-0008R-FITC

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: FITC

Catalog #1: bs-0008R-PE

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: PE

Catalog #1: bs-0008R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-0008R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-0011R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0011R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-0011R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0011R-Cy3

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-0011R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-0011R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-0011R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) 1:300 followed by conjugation to the secondary antibody and DAB staining
L1 mouse brain lysates L2 mouse lung lysates probed with Anti Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 26kD. Observed band size:26kD.
Image kindly submitted by an end-user via Funakoshi. 4% Paraformaldehyde fixed HeLa cells stained with Rabbit Anti-Cytochrome C Polyclonal Antibody (bs-0013R) at 1:100 for 2 hours at room temperature
HepG2 cells probed with Cytochrome C Polyclonal Antibody, unconjugated (bs-0013) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange).

Catalog #1: bs-0013R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0013R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Catalog #1: bs-0013R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0013R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-0013R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-0013R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: PE

Catalog #1: bs-0013R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-0013R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: FITC