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Primary Antibodies

Primary Antibodies

Our Primary Antibodies are research-use antibodies designed to support protein detection and target analysis across a wide range of applications and species. Our collection includes recombinant antibodies, monoclonal antibodies, and polyclonal antibodies for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and other common applications.

These primary antibodies are available for targets involved in cancer research, immunology, neuroscience, cell biology, metabolism, apoptosis, signaling pathways, and disease-related research. Bioss offers primary antibodies guaranteed for use in human, mouse, rat, and other species to help researchers choose the right antibody format for their experimental model and detection method.



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Catalog #1: bs-2622R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-2622R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Cy5

Catalog #1: bs-2622R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Cy3

Paraformaldehyde-fixed, paraffin embedded mouse tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with IL-22 Polyclonal Antibody (bs-2623R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Lane 1: A549 cell lysates probed with IL-22 Polyclonal Antibody, Unconjugated (bs-2623R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human 293T cell lysates; Lane 2: Human A549 cell lysates probed with IL-22 Polyclonal Antibody, Unconjugated (bs-2623R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2623R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-2623R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-2623R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-2624R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-2623R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bs-2623R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bs-2623R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: FITC

Lane 1: mouse liver lysates Lane 2: mouse intestine probed with Rabbit Anti-IL-22R Polyclonal Antibody, Unconjugated (bs-2624R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Mouse placenta lysates probed with IL-22R Polyclonal Antibody, Unconjugated (bs-2624R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2624R

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-2623R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bs-2624R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse

Conjugate: HRP

Mouse splenocytes probed with IL-22BP\/IL22RA2 Polyclonal Antibody, Unconjugated (bs-2625R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Mouse spleen lysates probed with IL22BP Polyclonal Antibody, unconjugated (bs-2625R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.\\n
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with IL-22BP\/IL22RA2 Polyclonal Antibody, Unconjugated (bs-2625R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Lane 1: Rat testis lysates; probed with IL-22BP\/IL22RA2 Polyclonal Antibody, unconjugated (bs-2625R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-2625R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2632R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-2631R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Mouse splenocytes probed with Rabbit Anti-IL-33 Polyclonal Antibody, PE-Cy5 Conjugated (bs-2633R-PE-Cy5) (green) at 1:100 for 40 minutes compared to control cells (blue).
Paraformaldehyde-fixed, paraffin embedded rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Anti \u2013IL-33 Polyclonal Antibody, Unconjugated (bs-2633R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary for 90 minutes and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-33 Polyclonal Antibody, Unconjugated (bs-2633R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-33 Polyclonal Antibody, Unconjugated (bs-2633R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2633R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2633R-Cy3

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-2633R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-2633R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Catalog #1: bs-2633R-Cy5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-2633R-Cy7

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-2633R-FITC

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: FITC

Lane 1: mouse heart lysates Lane 2: mouse brain lysates probed with Anti ERK1\/2(p42\/p42 MAPK) Polyclonal Antibody, Unconjugated (bs-2637R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 42kD. Observed band size: 42kD.
A549 cell lysates probed with Rabbit Anti-ERK1 + 2 Polyclonal Antibody, Unconjugated (bs-2673R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Lane 1: Mouse Kidney lysates; Lane 2: Mouse Liver lysates probed with ERK1 + 2 Polyclonal Antibody, Unconjugated (bs-2637R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: A431 cell Lysate;Lane 2: Mouse Cerebrum Lysate;Lane 3: Rat Cerebrum Lysate;Lane 4: Mouse Lymph node Lysate;Lane 5: Rat Lymph node Lysate;Lane 6: SiHa cell Lysate;Lane 7: HUVEC cell Lysate probed with ERK1 + ERK2 Polyclonal Antibody, Unconjugated (bs-2637R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C\r\n\r\n

Catalog #1: bs-2637R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2637R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-2637R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-2637R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-2637R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-2637R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-2637R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-2640R

Applications: IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Lane 1: SW480 cell lysates; Lane 2: A549 cell lysates; Lane 3: Huvec cell lysates; Lane 4: A431 cell lysates probed with Integrin alpha 6 Polyclonal Antibody, Unconjugated (bs-2641R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human SW480 cell lysates; Lane 2: Human A549 cell lysates; Lane 3: Human HUVEC cell lysates; Lane 4: Human A431 cell lysates probed with Integrin alpha 6 Polyclonal Antibody, Unconjugated (bs-2641R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2641R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-2641R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-2641R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-2642R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-2642R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-2642R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-2642R-Cy3

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy3

Catalog #1: bs-2642R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy5

Catalog #1: bs-2642R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy7

Catalog #1: bs-2643R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-2642R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: FITC

Catalog #1: bs-2643R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-2643R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-2643R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-2643R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-2643R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-2644R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated