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Primary Antibodies



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25 ug total protein per lane of various lysates (see on figure) probed with c-Myc monoclonal antibody, unconjugated (bsm-56001R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-c-Myc antibody (bsm-56001R): 1 \u03bcg\/10^6 cells; Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (c-Myc) monoclonal Antibody, unconjugated (bsm-56001R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Catalog #1: bsm-56001r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-61135r-percp-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-61135r-pe-cy3

Applications: WB

Reactivity: Human

Conjugate: PE-Cy3

Catalog #1: bsm-61135r-apc-cy5

Applications: WB, IF(ICC)

Reactivity: Human

Conjugate: APC-Cy5

Catalog #1: bsm-30109m-biotin-100ul

Applications: IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-30109m-hrp-100ul

Applications: IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-56001r-bf555-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-56001r-apc-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-56001r-bf488-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-56001r-apc-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bsm-56001r-cy5-100ul

Applications: WB

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-56001r-bf647-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-56001r-bf405-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-56001r-bf680-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-56001r-apc-100ul

Applications: WB

Reactivity: Human

Conjugate: APC

Catalog #1: bsm-56001r-bf750-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-56001r-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-56001r-biotin-100ul

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-56001r-pe-100ul

Applications: WB

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-56001r-bf594-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-56001r-bf350-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-56001r-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-56001r-cy3-100ul

Applications: WB

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-56001r-hrp-100ul

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-56001r-fitc-100ul

Applications: WB

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-56001r-percp-100ul

Applications: WB

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-56001r-pe-cy5-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bsm-56001r-percp-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-56001r-pe-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bsm-56001r-pe-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-56001r-percp-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-56001r-rbitc-100ul

Applications: WB

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-33002m

Applications: WB, ELISA

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bsm-33002m-bf350-100ul

Applications: WB

Reactivity: Species independent

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33002m-bf488-100ul

Applications: WB

Reactivity: Species independent

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33002m-bf555-100ul

Applications: WB

Reactivity: Species independent

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33002m-apc-cy7-100ul

Applications: WB

Reactivity: Species independent

Conjugate: APC-Cy7

Catalog #1: bsm-33002m-apc-Cy5.5-100ul

Applications: WB

Reactivity: Species independent

Conjugate: APC-Cy5.5

Catalog #1: bsm-33002m-bf405-100ul

Applications: WB

Reactivity: Species independent

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33002m-bf647-100ul

Applications: WB

Reactivity: Species independent

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33002m-bf594-100ul

Applications: WB

Reactivity: Species independent

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33002m-bf750-100ul

Applications: WB

Reactivity: Species independent

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33002m-apc-100ul

Applications: WB

Reactivity: Species independent

Conjugate: APC

Catalog #1: bsm-33002m-cy3-100ul

Applications: WB

Reactivity: Species independent

Conjugate: Cy3

Catalog #1: bsm-33002m-biotin-100ul

Applications: WB, ELISA

Reactivity: Species independent

Conjugate: Biotin

Catalog #1: bsm-33002m-bf680-100ul

Applications: WB

Reactivity: Species independent

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33002m-pe-cy5-100ul

Applications: WB

Reactivity: Species independent

Conjugate: PE-Cy5

Catalog #1: bsm-33002m-cy5-100ul

Applications: WB

Reactivity: Species independent

Conjugate: Cy5