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Primary Antibodies

Primary Antibodies

Our Primary Antibodies are research-use antibodies designed to support protein detection and target analysis across a wide range of applications and species. Our collection includes recombinant antibodies, monoclonal antibodies, and polyclonal antibodies for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and other common applications.

These primary antibodies are available for targets involved in cancer research, immunology, neuroscience, cell biology, metabolism, apoptosis, signaling pathways, and disease-related research. Bioss offers primary antibodies guaranteed for use in human, mouse, rat, and other species to help researchers choose the right antibody format for their experimental model and detection method.



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Catalog #1: bsm-51062M

Applications: WB

Reactivity: Human, Other Species

Conjugate: Unconjugated

Catalog #1: bsm-51111M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51188M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with ER alpha (5G7) Monoclonal Antibody (bsm-53007M) at a concentration of 7 \u03bcg\/ ml. Figure shows that the antibody is specific for ER alpha isoform, whereas the ER beta isoform is not recognized.
COS-7 cells transiently over-expressing human ER\u03b1 (left) or ER\u00df1 (right) were labeled with the ER alpha (5G7) Monoclonal Antibody (bsm-53007M), used at a concentration of 15 \u03bcg\/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure shows the specificity of the antibody for the ER alpha isoform.
ChIP assays was performed using MCF7 cells treated with the ER agonist estradiol for 3 hours prior to harvesting, ER alpha (5G7) Monoclonal Antibody (bsm-53007M), and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 \u03bcg of antibody were used per ChIP experiment. Recovery (%: ChIP\/input) and occupancy (x fold: +ve\/-ve) are shown. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene used as a negative control. Figure shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +\/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.

Catalog #1: bsm-53007M

Applications: WB, IHC-P, IHC, dot-blot

Reactivity: Human, Monkey

Conjugate: Unconjugated

Western blot was performed on whole cell extracts from HeLa cells (25 \u03bcg, lane 1) and recombinant histone H2A, H2B, H3 and H4 (1ug, lane 2, 3, 4 and 5, respectively) using the H3pan (1B2) Monoclonal Antibody (bsm-53008M). The antibody was diluted 1:1,000 in TBSTween containing 5% skimmed milk, followed by secondary antibody incubation and exposure.
Western blot was performed on whole cell extracts (30\u03bcg) from different cell types (lane 1: HeLa, lane 2: K562, lane 3: MCF7, lane 4: U2OS, lane 5: HepG2, lane 6: Jurkat, lane 7: NIH3T3, lane 8: E14Tg2a mouse ES cells) using H3pan Monoclonal Antibody (bsm-53008M) at 1:1,000 dilution in TBS-Tween containing 5% skimmed milk, followed by secondary antibody incubation and exposure.
HeLa cells stained with the H3pan Monoclonal Antibody (bsm-53008M) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 1% BSA. The cells were labeled with the H3 antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

Catalog #1: bsm-53008M

Applications: WB, IF(ICC)

Reactivity: Human, Others

Conjugate: Unconjugated

Catalog #1: bsm-51530m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51008r-50ug

Applications: WB

Reactivity: Human, Others

Conjugate: Unconjugated

Catalog #1: bsm-41636M

Applications: ELISA

Reactivity: Virus

Conjugate: Unconjugated

Catalog #1: bsm-72017m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72032m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72087m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72122m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72129m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72138m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72183m

Applications: ELISA, IHC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72279m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72280m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72399m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72404m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72430m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72431m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72433m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72434m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72441m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72442m

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72453m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72455m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72457m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-41907m-bf350-100ul

Applications: IF

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-41907m-apc-100ul

Applications: IF

Reactivity: Human

Conjugate: APC

Catalog #1: bsm-41907m-bf647-100ul

Applications: IF

Reactivity: Human

Conjugate: AbBy Fluor-647

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CA19-9 Monoclonal Antibody, Unconjugated (bsm-41907M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CA19-9 Monoclonal Antibody, Unconjugated (bsm-41907M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CA19-9 Monoclonal Antibody, Unconjugated (bsm-41907M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CA19-9 Monoclonal Antibody, Unconjugated (bsm-41907M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-41907m

Applications: IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-41907m-bf488-100ul

Applications: IF

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-41907m-cy3-100ul

Applications: IF

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-41907m-bf405-100ul

Applications: IF

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-41907m-biotin-100ul

Applications: IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-41907m-apc-Cy5.5-100ul

Applications: IF

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-41907m-Cy5.5-100ul

Applications: IF

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-41907m-cy5-100ul

Applications: IF

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-41907m-bf555-100ul

Applications: IF

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-41907m-bf594-100ul

Applications: IF

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-41907m-percp-Cy5.5-100ul

Applications: IF

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-41907m-bf750-100ul

Applications: IF

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-41907m-pe-100ul

Applications: IF

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-41907m-fitc-100ul

Applications: IF

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-41907m-hrp-100ul

Applications: IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-41907m-percp-100ul

Applications: IF

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-41907m-bf680-100ul

Applications: IF

Reactivity: Human

Conjugate: AbBy Fluor-680