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Primary Antibodies



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Catalog #1: bs-2224R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-2224R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bs-2224R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bs-2224R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5

Human A549 lysates probed with p-Smad3(Ser423) Polyclonal Antibody, Unconjugated (bs-2225R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
HUVEC cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Smad3 (Ser423) Polyclonal Antibody(bs-2225R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Heart lysates; Lane 2: Mouse Kidney lysates; Lane 3: Rat Cerebrum lysates probed with Smad3 Polyclonal Antibody, Unconjugated (bs-2225R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-Smad3 (Ser423)) polyclonal Antibody, Unconjugated (bs-2225R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-2225R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2224R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bs-2225R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-2225R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-2225R-Cy5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-2225R-Cy3

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-2225R-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-2225R-FITC

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-2231R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-2231R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-2231R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-2231R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-2231R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-2231R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-2231R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-2232R

Applications: ELISA, IHC-P, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bs-2232R-Biotin

Applications: ELISA, IHC-P

Reactivity: Others

Conjugate: Biotin

Catalog #1: bs-2232R-HRP

Applications: ELISA, IHC-P

Reactivity: Others

Conjugate: HRP

Catalog #1: bs-2232R-Cy3

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: Cy3

Catalog #1: bs-2239R

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2232R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: Cy5

Catalog #1: bs-2239R-HRP

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-2232R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: Cy7

Catalog #1: bs-2232R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: FITC

Catalog #1: bs-2241R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rabbit alveolar bone labeled with Anti-BMP7 Polyclonal Antibody, Unconjugated (bs-2242R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rabbit alveolar bone tissue labeled with Anti-BMP7 Polyclonal Antibody, Unconjugated (bs-2242R) at 1:300 followed by conjugation to the secondary antibody
Mouse bone lysates probed with BMP7 Polyclonal Antibody, Unconjugated (bs-2242R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Kidney lysates; Lane 2: Mouse Placenta lysates; Lane 3: Mouse Cerebrum lysates; Lane 4: Mouse NIH\/3T3 cell lysates; Lane 5: Rat Cerebrum lysates probed with BMP7 Polyclonal Antibody, Unconjugated (bs-2242R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2242R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-2242R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Biotin

Catalog #1: bs-2242R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: HRP

Catalog #1: bs-2242R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Cy7

Catalog #1: bs-2242R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Cy5

Catalog #1: bs-2242R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Cy3

Catalog #1: bs-2242R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: FITC

Catalog #1: bs-2247R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-2247R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-2247R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-2247R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy5

Catalog #1: bs-2247R-Cy3

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy3

Catalog #1: bs-2249R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-2249R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-2247R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy7

Catalog #1: bs-2249R-Cy3

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy3

Catalog #1: bs-2249R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy5

Catalog #1: bs-2249R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-2249R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy7