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Primary Antibodies



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Catalog #1: bs-0367G-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy3

Catalog #1: bs-0367G-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy5

Catalog #1: bs-0372R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-0367G-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy7

Catalog #1: bs-0367G-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: FITC

Catalog #1: bs-0372R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-0372R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-0372R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy3

Catalog #1: bs-0376R

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0372R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy5

Catalog #1: bs-0372R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy7

Catalog #1: bs-0372R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: FITC

Catalog #1: bs-0376R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0376R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-0379R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0379R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0379R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Catalog #1: bs-0379R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-0379R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-0379R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-0380R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-0379R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: FITC

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-MBP Polyclonal Antibody (bs-0380R), Unconjugated at 1:200, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, PE conjugated (bs-0295G-PE) at 1:200 for 40 minutes at 37\u00b0C DAPI(5ug\/ml, blue) was used to stain the cell nuclei
Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-MBP Polyclonal Antibody (bs-0380R), Unconjugated at 1:200, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, PE conjugated (bs-0295G-PE) at 1:200 for 40 minutes at 37\u00b0C
A549 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MBP Antibody(bs-0380R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (bs-0380R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0380r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0380R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0380R-Cy3

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-0380R-Cy5

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-0380R-Cy7

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-0380R-FITC

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-0381R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-ARC Polyclonal Antibody (bs-0385R), Unconjugated at 1:200, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, PE conjugated (bs-0295G-PE) at 1:200 for 40 minutes at 37\u00b0C DAPI(5ug\/ml, blue) was used to stain the cell nuclei
A549 cells probed with Rabbit Anti-ARC\/Arg3.1 Polyclonal Antibody (bs-0385R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary.\\n
Lane 1: Rat brain; Lane 2: mouse brain lysate probed with ARC Polyclonal Antibody, Unconjugated (bs-0385R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0385R

Applications: WB, ELISA, FCM

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0385R-Biotin

Applications: WB, ELISA

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0385R-HRP

Applications: WB, ELISA

Reactivity: Mouse, Rat

Conjugate: HRP

Catalog #1: bs-0385R-Cy3

Applications: WB, FCM

Reactivity: Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-0385R-Cy5

Applications: WB, FCM

Reactivity: Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-0385R-Cy7

Applications: WB, FCM

Reactivity: Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-0385R-PE

Applications: WB, FCM

Reactivity: Mouse, Rat

Conjugate: PE

Catalog #1: bs-0385R-FITC

Applications: WB, FCM

Reactivity: Mouse, Rat

Conjugate: FITC

Catalog #1: bs-0392R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-0392R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-0392R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-0392R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy7

Catalog #1: bs-0392R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy3

Catalog #1: bs-0392R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy5

Catalog #1: bs-0392R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: FITC

Paraformaldehyde-fixed, paraffin embedded Human Cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HOXc8 Polyclonal Antibody, Unconjugated (bs-0394R) at 1:500 overnight at 4\u00b0C, DAB staining.
Rat liver lysates probed with HOXc8 Polyclonal Antibody, Unconjugated (bs-0394R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0394R

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Lane 1: Human VCAM1 protein; Probed with Rabbit Anti-VCAM1 Polyclonal Antibody, Unconjugated (bs-0396R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:20000 for 90 min at 37˚C.
Mouse spleen cells (dark blue) were fixed with 2% paraformaldehyde for 10 min at 37\u2103. Primary antibody, Rabbit Anti-VCAM-1 antibody (bs-0396R, Green) at 1\u03bcg \/8x10^5 cells was incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (1 hour) to block non-specific protein-protein interactions. Then Goat Anti-rabbit IgG\/FITC (light blue) antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1\/200 dilution for 40 min at room temperature. Isotype control Rabbit IgG (orange) was analyzed under the same conditions.
Lane 1: Human Siha cell lysates; Lane 2: Human HUVEC cell lysates; Lane 3: Human U251 cell lysates probed with VCAM-1 Polyclonal Antibody, Unconjugated (bs-0396R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Spleen tissue lysates; Lane 3: Mouse Cerebrum tissue lysates probed with VCAM1 Polyclonal Antibody, Unconjugated (bs-0396R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0396r-TR

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded rat brain labeled with Anti-Deltex 1 Polyclonal Antibody, Unconjugated(bs-0399R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.DAPI was used to stain the cell nuclei
Antigen: bs-0399P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Lane 1: Jurkat cell lysates probed with Deltex 1 Polyclonal Antibody, Unconjugated (bs-0399R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0399R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0399R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin