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Bcl-2 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat kidney tissue labeled withAnti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032r) 1:400 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat kidney tissue labeled withAnti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032r) 1:400 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) 1:400 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) 1:400 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat myocardium tissue labeled with Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) 1:300 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat myocardium tissue labeled with Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) 1:300 followed by conjugation to the secondary antibody and DAB staining
L1 mouse embryo lysates, L2 mouse brain lysates probed with Anti- Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. P
L1 mouse embryo lysates, L2 mouse brain lysates probed with Anti- Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. P
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bcl-2) polyclonal Antibody, Unconjugated (bs-0032R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bcl-2) polyclonal Antibody, Unconjugated (bs-0032R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Human MCF-7 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human HL-60 cell lysates; Lane 4: Human A431 cell lysates probed with Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human MCF-7 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human HL-60 cell lysates; Lane 4: Human A431 cell lysates probed with Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Lymph node tissue lysates; Lane 2: Mouse Bone tissue lysates; Lane 3: Mouse Placenta tissue lysates; Lane 4: Rat Spleen tissue lysates; Lane 5: Rat Placenta tissue lysates; Lane 6: Human K562 cell lysates; Lane 7: Human A549 cell lysates probed with Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Lymph node tissue lysates; Lane 2: Mouse Bone tissue lysates; Lane 3: Mouse Placenta tissue lysates; Lane 4: Rat Spleen tissue lysates; Lane 5: Rat Placenta tissue lysates; Lane 6: Human K562 cell lysates; Lane 7: Human A549 cell lysates probed with Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
25 ug total protein per lane of various lysates (see on figure) probed with Bcl-2 polyclonal antibody, unconjugated (bs-0032R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Bcl-2 polyclonal antibody, unconjugated (bs-0032R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Chicken
Overview
Catalog # bs-0032r
Product Name Bcl-2 Polyclonal Antibody
Applications
WB ELISA IHC-P IHC-F IF
Reactivity Human, Mouse, Rat
Predicted Reactivity Chicken
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Bcl-2
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 596
Swiss Prot P10415
Subcellular location Cytoplasm, Nucleus, Cell membrane
Synonyms Apoptosis regulator Bcl 2; Apoptosis regulator Bcl2; AW986256; B cell CLL/lymphoma 2; B cell leukemia/lymphoma 2; B cell lymphoma 2; Bcl 2; Bcl-2; Bcl2; BCL2 protein; C430015F12Rik; D630044D05Rik; D830018M01Rik; Leukemia/lymphoma, B-cell, 2; Oncogene B-cell leukemia 2; BCL2_HUMAN.
Application Dilution
WB =1:500-2000
ELISA ELISA=1:5000-10000
IHC-P IHC-P=1:100-500
IHC-F IHC-F=1:100-500
IF IF=1:100-500

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