NMDAR2B Polyclonal Antibody

$Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-NR2B\/NMDAR2B Polyclonal Antibody, Unconjugated (bs-0222R) at 1:300, followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-NR2B\/NMDAR2B Polyclonal Antibody, Unconjugated (bs-0222R) at 1:300, followed by conjugation to the secondary antibody and DAB staining

$Formalin-fixed and paraffin embedded: : rat brain tissue labeled with Anti-NR2B\/NMDAR2B Polyclonal Antibody, Unconjugated (bs-0222R) at 1:200,, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC) at 1:200 for 40 minutes at 37\u00b0C and DAPI staining$

Formalin-fixed and paraffin embedded: : rat brain tissue labeled with Anti-NR2B\/NMDAR2B Polyclonal Antibody, Unconjugated (bs-0222R) at 1:200,, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC) at 1:200 for 40 minutes at 37\u00b0C and DAPI staining

$Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-NR2B\/NMDAR2B Polyclonal Antibody, Unconjugated (bs-0222R) at 1:200,, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC) at 1:200 for 40 minutes at 37\u00b0C$

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-NR2B\/NMDAR2B Polyclonal Antibody, Unconjugated (bs-0222R) at 1:200,, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC) at 1:200 for 40 minutes at 37\u00b0C

$A549 lysates probed with NMDAR2B Polyclonal Antibody, Unconjugated (bs-0222R) at 1:300 overnight at 4\u02daC. Followed by a conjugated secondary antibody at 1:10000 for 60 min at 37\u02daC.$

A549 lysates probed with NMDAR2B Polyclonal Antibody, Unconjugated (bs-0222R) at 1:300 overnight at 4\u02daC. Followed by a conjugated secondary antibody at 1:10000 for 60 min at 37\u02daC.

$Paraformaldehyde-fixed, paraffin embedded Rat Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NR2B Polyclonal Antibody, Unconjugated (bs-0222R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NR2B Polyclonal Antibody, Unconjugated (bs-0222R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Rat

Predicted Reactivity

Mouse

Overview
Catalog #	bs-0222r
Product Name	NMDAR2B Polyclonal Antibody
Applications	WB ELISA FCM IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human, Rat
Predicted Reactivity	Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human NMDAR2B
Immunogen Range	451-550/1484
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2904
Swiss Prot	Q13224
Subcellular location	Cell membrane
Synonyms	MRD6; NR2B; hNR3; GluN2B; NMDAR2B; Glutamate receptor ionotropic, NMDA 2B; Glutamate [NMDA] receptor subunit epsilon-2; N-methyl D-aspartate receptor subtype 2B; N-methyl-D-aspartate receptor subunit 3; NR3; GRIN2B
Background	NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death (By similarity).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200