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His tag Polyclonal Antibody

His-Tagged Fusion Protein Overexpression E.coli Lysates (Cat#: bs-41230P) probed with His tag Polyclonall Antibody, Unconjugated (bs-0287R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
His-Tagged Fusion Protein Overexpression E.coli Lysates (Cat#: bs-41230P) probed with His tag Polyclonall Antibody, Unconjugated (bs-0287R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
His-Tagged Fusion Protein Overexpression E.coli Lysates (Cat#: bs-41403P) probed with His tag Polyclonall Antibody, Unconjugated (bs-0287R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
His-Tagged Fusion Protein Overexpression E.coli Lysates (Cat#: bs-41403P) probed with His tag Polyclonall Antibody, Unconjugated (bs-0287R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • IHC-P

Reactivity

  • Species independent
Overview
Catalog # bs-0287R
Product Name His tag Polyclonal Antibody
Applications
WB IHC-P
Reactivity Species independent
Specifications
Conjugation Unconjugated
Host Rabbit
Source synthetic peptide: HHHHHH conjugated to KLH
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Synonyms HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.
Background The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400

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