CD45 Polyclonal Antibody

Applications

  • WB
  • ELISA
  • FCM
  • IHC-P
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
  • Rabbit

Predicted Reactivity

  • Cow
Overview
Catalog # bs-0522R
Product Name CD45 Polyclonal Antibody
Applications WB, ELISA, FCM, IHC-P, IF(IHC-P), IF(ICC)
Specificity The antibody is predicted to work with Mouse and Rat samples, but with decreased sensitivity compared to human samples.
Reactivity Human, Mouse, Rat, Rabbit
Predicted Reactivity Cow
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CD45
Immunogen Range 1210-1304/1304
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 5788
Swiss Prot P08575
Subcellular location Cell membrane
Synonyms LCA; LY5; B220; CD45; L-CA; T2; CD45R; GP18; Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; PTPRC
Background Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200