CaMK2 alpha Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-CaMK2a Polyclonal Antibody (bs-0564R), Unconjugatedat 1:200, followed by conjugation to the secondary antibody and DAB staining

$MCF-7 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with CaMK2 alpha Polyclonal Antibody(bs-0564R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).$

MCF-7 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with CaMK2 alpha Polyclonal Antibody(bs-0564R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Pig
Chicken
Guinea Pig

Overview
Catalog #	bs-0564R
Product Name	CaMK2 alpha Polyclonal Antibody
Applications	WB ELISA FCM IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Pig, Chicken, Guinea Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CaMK2a
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	815
Subcellular location	Cell membrane
Synonyms	alpha CaMKII; Calcium / Calmodulin Dependent Protein Kinase II G; Calcium calmodulin dependent protein kinase CaM kinase II alpha; Calcium calmodulin dependent protein kinase II alpha-B subunit; Calcium calmodulin dependent protein kinase II; Calcium/calmodulin dependent protein kinase II alpha; Calcium/calmodulin-dependent protein kinase CaM kinase II alpha; Calcium/calmodulin-dependent protein kinase II alpha; Calcium/calmodulin-dependent protein kinase II-alpha; Calcium/calmodulin-dependent protein kinase type II subunit alpha; Calcium/calmodulin-dependent protein kinase type IIA; CaM kinase II alpha chain; CaM kinase II alpha subunit; CaM kinase II subunit alpha; CAM2; CaMK II alpha subunit; CaMK-II subunit alpha; CAMK2A; CAMKA; CAMKB; CaMKII; CaMK 2a; CaMK2a; KCC2A_HUMAN.
Background	The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008].
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200