Pokemon/ZBTB7A Polyclonal Antibody

Formalin-fixed and paraffin embedded human colon carcinoma with Anti-Pokemon Polyclonal Antibody, Unconjugated (bs-0891R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human gastric carcinoma with Anti-Pokemon Polyclonal Antibody, Unconjugated (bs-0891R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

$293T lysates probed with PokemonZBTB7A Polyclonal Antibody, Unconjugated (bs-0891R) at 1:500 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37\u02daC.$

293T lysates probed with PokemonZBTB7A Polyclonal Antibody, Unconjugated (bs-0891R) at 1:500 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37\u02daC.

$25 ug total protein per lane of various lysates (see on figure) probed with Pokemon\/ZBTB7A polyclonal antibody, unconjugated (bs-0891R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with Pokemon\/ZBTB7A polyclonal antibody, unconjugated (bs-0891R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse

Predicted Reactivity

Human
Rat
Dog
Cow
Sheep
Pig
Chicken

Overview
Catalog #	bs-0891R
Product Name	Pokemon/ZBTB7A Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Mouse
Predicted Reactivity	Human, Rat, Dog, Cow, Sheep, Pig, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Pokemon
Immunogen Range	151-250/569
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	51341
Swiss Prot	O95365
Subcellular location	Nucleus
Synonyms	LRF; FBI1; FBI-1; ZBTB7; ZNF857A; pokemon; Zinc finger and BTB domain-containing protein 7A; Factor binding IST protein 1; Factor that binds to inducer of short transcripts protein 1; HIV-1 1st-binding protein 1; Leukemia/lymphoma-related factor; POZ and Krueppel erythroid myeloid ontogenic factor; POK erythroid myeloid ontogenic factor; TTF-I-interacting peptide 21; TIP21; Zinc finger protein 857A; ZBTB7A
Background	Plays a key role in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals (By similarity). Specifically represses the transcription of the CDKN2A gene. Efficiently abrogates E2F1-dependent CDKN2A transactivation/de-repression. Binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3' (By similarity).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200