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GRK1 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Anti-GRK1 Polyclonal Antibody, Unconjugated (bs-1082R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-GRK1 Polyclonal Antibody, Unconjugated (bs-1082R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse lung labeled with Anti-GRK1 Polyclonal Antibody, Unconjugated (bs-1082R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse lung labeled with Anti-GRK1 Polyclonal Antibody, Unconjugated (bs-1082R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GRK1 Polyclonal Antibody, Unconjugated (bs-1082R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GRK1 Polyclonal Antibody, Unconjugated (bs-1082R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Human HUVEC cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human Ramos cell lysates probed with GRK1 Polyclonal Antibody, Unconjugated (bs-1082R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human HUVEC cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human Ramos cell lysates probed with GRK1 Polyclonal Antibody, Unconjugated (bs-1082R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • ELISA

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Cow
  • Chicken
Overview
Catalog # bs-1082R
Product Name GRK1 Polyclonal Antibody
Applications WB, ELISA
Reactivity Human, Mouse, Rat
Predicted Reactivity Cow, Chicken
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human GRK1
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 6011
Swiss Prot Q15835
Subcellular location Cell membrane
Synonyms G-protein coupled receptor kinase 1; GRK1; GPRK1; RK; Grk1; Rhok; RHODOPSIN KINASE; RK_HUMAN.
Application Dilution
WB =1:500-2000
ELISA ELISA=1:5000-10000

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