CHI3L1 Polyclonal Antibody

$L1 mouse embryo lysates L2 mouse liver lysates probed with Anti CGP-39\/YKL-40 Polyclonal Antibody, Unconjugated (bs-1093R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 42kD. Observed band size: 42kD$

L1 mouse embryo lysates L2 mouse liver lysates probed with Anti CGP-39\/YKL-40 Polyclonal Antibody, Unconjugated (bs-1093R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 42kD. Observed band size: 42kD

Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-CHI3L1 Polyclonal Antibody, Unconjugated (bs-1093R) at 1:300 followed by conjugation to the secondary antibody and DAB staining

$U87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (CHI3L1) polyclonal Antibody, Unconjugated (bs-1093R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

U87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (CHI3L1) polyclonal Antibody, Unconjugated (bs-1093R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

$Lane 1: Mouse Lung tissue lysates; Lane 2: Rat Lung tissue lysates probed with CHI3L1 Polyclonal Antibody, Unconjugated (bs-1093R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Lung tissue lysates; Lane 2: Rat Lung tissue lysates probed with CHI3L1 Polyclonal Antibody, Unconjugated (bs-1093R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IHC-F

Reactivity

Human

Predicted Reactivity

Mouse
Rat

Overview
Catalog #	bs-1093R
Product Name	CHI3L1 Polyclonal Antibody
Applications	WB IHC-P IHC-F
Reactivity	Human
Predicted Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from rat CGP-39/CHI3L1
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	89824
Subcellular location	Secreted, Extracellular matrix
Synonyms	Chitinase-3-like protein 1; Cartilage glycoprotein 39; CGP-39; GP-39; Chi3l1
Background	Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung (By similarity).
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500