RhoA/C Polyclonal Antibody, HRP Conjugated

Applications

WB
ELISA
IHC-P
IHC-F

Reactivity

Human
Mouse
Rat
Zebrafish

Predicted Reactivity

Dog
Chicken
Rabbit

Overview
Catalog #	bs-1180R-HRP
Product Name	RhoA/C Polyclonal Antibody, HRP Conjugated
Applications	WB, ELISA, IHC-P, IHC-F
Reactivity	Human, Mouse, Rat, Zebrafish
Predicted Reactivity	Dog, Chicken, Rabbit
Specifications
Conjugation	HRP
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human RhoA
Immunogen Range	101-193/193
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID	387, 389
Swiss Prot	P61586
Subcellular location	Cytoplasm, Cell membrane
Synonyms	ARHA; ARH12; RHO12; RHOH12; Transforming protein RhoA; Rho cDNA clone 12; h12; RHOA; Rho-related GTP-binding protein RhoC; Rho cDNA clone 9; ARH9, ARHC.
Background	Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500

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