phospho-PDCD4 (Ser67) Polyclonal Antibody

$HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-PDCD4 (Ser67)) polyclonal Antibody, Unconjugated (bs-12594R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-PDCD4 (Ser67)) polyclonal Antibody, Unconjugated (bs-12594R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

IHC-P
IHC-F
IF
Flow-Cyt
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-12594R
Product Name	phospho-PDCD4 (Ser67) Polyclonal Antibody
Applications	IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthesised phosphopeptide derived from human PDCD4 around the phosphorylation site of Ser67
Modification Site	Ser67
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	27250
Swiss Prot	Q53EL6
Synonyms	PDCD4 (phospho S67); p-PDCD4 (phospho S67); Death up-regulated gene protein; Dug; H731; Ma3; MGC33046; MGC33047; Neoplastic transformation inhibitor; Neoplastic transformation inhibitor protein; Nuclear antigen H731; Nuclear antigen H731 like; Nuclear antigen H731 like protein; Nuclear antigen H731-like; PDCD 4; Pdcd4; PDCD4_HUMAN; Programmed cell death 4; programmed cell death 4 (neoplastic transformation inhibitor); Programmed cell death protein 4; Protein 197/15a; Protein MA-3; Tis; Topoisomerase-inhibitor suppressed protein.
Background	This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]
Application Dilution
IHC-P
IHC-F	1:100-500
IF	ICC1:50-200
Flow-Cyt	IF
ICC/IF