AIF1/Iba1 Polyclonal Antibody

Formalin-fixed and paraffin-embedded rat brain labeled with AIF1 Polyclonal Antibody at 1:200 dilution followed by incubation with secondary antibody and DAB staining

Image by provided by the Independent Validation program, badge number 029613:Frozen,OCT embedded mouse spleen labeled with Anti-AIF1 Polyclonal Antibody, Unconjugated (bs-1363R) at 1:100 followed by conjugation to the secondary antibody and DAPI staining

$Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-AIF1 Polyclonal Antibody, unonjugated (bs-1363R) at 1:200 for 40 minutes at 37\u00b0C followed by labeling Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3) 1:200, 40 minutes at 37\u00b0C$

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-AIF1 Polyclonal Antibody, unonjugated (bs-1363R) at 1:200 for 40 minutes at 37\u00b0C followed by labeling Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3) 1:200, 40 minutes at 37\u00b0C

$Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-AIF1 Polyclonal Antibody, Unconjugated (bs-1363R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.\\n$

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-AIF1 Polyclonal Antibody, Unconjugated (bs-1363R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.\\n

$Formalin-fixed and paraffin embedded mouse spinal cord stained with AIF1 Polyclonal Antibody (bs-1363R) at 1:100 dilution. (a) Sham group; (b) Tumor group; (c) l-CDL 5 mg\/kg group; (d) l-CDL 10 mg\/kg group; (e) l-CDL 20 mg\/kg group; (f) O&A 1&65 m\/kg group (g) l-THP 20 mg\/kg group. B Graph showing the mean fluorescence intensity for AIF1.$

Formalin-fixed and paraffin embedded mouse spinal cord stained with AIF1 Polyclonal Antibody (bs-1363R) at 1:100 dilution. (a) Sham group; (b) Tumor group; (c) l-CDL 5 mg\/kg group; (d) l-CDL 10 mg\/kg group; (e) l-CDL 20 mg\/kg group; (f) O&A 1&65 m\/kg group (g) l-THP 20 mg\/kg group. B Graph showing the mean fluorescence intensity for AIF1.

$BV-microglia stained with AIF1 Polyclonal Antibody (bs-1363R) at 1:100 dilution. AIF1 expression (green) in activated BV-microglia is observed using confocal microscopy. The blue staining represents DAPI. (a) Control; (b) treated with CCL2 (3 ng\/mL); (c) CCL2 (3 ng\/mL) + l-CDL (3 \u00b5M); (d) CCL2 (3 ng\/mL) + l-CDL (10 \u00b5M); (e) CCL2 (3 ng\/mL) + l-CDL (30 \u00b5M); (f) CCL2 (3 ng\/mL) + RS504393 (10 \u00b5M);$

BV-microglia stained with AIF1 Polyclonal Antibody (bs-1363R) at 1:100 dilution. AIF1 expression (green) in activated BV-microglia is observed using confocal microscopy. The blue staining represents DAPI. (a) Control; (b) treated with CCL2 (3 ng\/mL); (c) CCL2 (3 ng\/mL) + l-CDL (3 \u00b5M); (d) CCL2 (3 ng\/mL) + l-CDL (10 \u00b5M); (e) CCL2 (3 ng\/mL) + l-CDL (30 \u00b5M); (f) CCL2 (3 ng\/mL) + RS504393 (10 \u00b5M);

$Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (AIF1) Polyclonal Antibody, Unconjugated (bs-1363R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (AIF1) Polyclonal Antibody, Unconjugated (bs-1363R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-1363R
Product Name	AIF1/Iba1 Polyclonal Antibody
Applications	WB ELISA FCM IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human AIF1
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	199
Swiss Prot	P55008
Subcellular location	Cytoplasm, Cell membrane
Synonyms	IBA1; IRT1; AIF-1; IRT-1; Allograft inflammatory factor 1; Ionized calcium-binding adapter molecule 1; Protein G1; AIF1; G1
Background	Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200