GRAP Polyclonal Antibody

$Lane 1: Mouse Prostate tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Human A549 cell lysates; Lane 5: Human MCF-7 cell lysates; Lane 6: Human HeLa cell lysates probed with GRAP Polyclonal Antibody, Unconjugated (bs-13640R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.$

Lane 1: Mouse Prostate tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Human A549 cell lysates; Lane 5: Human MCF-7 cell lysates; Lane 6: Human HeLa cell lysates probed with GRAP Polyclonal Antibody, Unconjugated (bs-13640R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

Applications

ELISA

Predicted Reactivity

Human
Mouse
Rat
Cow
Horse
Rabbit

Overview
Catalog #	bs-13640r-100ul
Product Name	GRAP Polyclonal Antibody
Applications	ELISA
Predicted Reactivity	Human, Mouse, Rat, Cow, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human GRAP
Immunogen Range	51-150/217
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Subcellular location	Cytoplasm
Synonyms	Grap; GRAP_HUMAN; GRB2 related adaptor protein; GRB2-related adapter protein; Growth factor receptor bound protein 2 related adaptor protein; MGC64880; OTTHUMP00000065766.
Background	Many growth factors function by binding receptors with intrinsic tyrosine kinase activity. Signaling by such receptors involves a series of intermediates characterized by SH2 domains that bind tyrosine phosphorylated receptors by a direct interaction between the SH2 domain and specific receptor sequences. For instance, the GRB family of proteins lack a defined catalytic activity and are entirely composed of SH2 and SH3 domains. Members include GRB2, GRB7, GRB10 (also referred to as GRB-IR), GRB14 and Grap (for GRB2-related adapter protein). While GRB10 and GRB14 are most closely related to GRB7, Grap shares the highest degree of homology with GRB2 exhibiting 59% sequence identity with GRB2. The Grap SH2 domain is capable of binding to the activated stem cell factor receptor, c-Kit and the erythropoietin receptor (EpoR). Grap also associates with the Ras guanine nucleotide exchange factor Sos 1 via its amino terminal SH3 domain.
Application Dilution
ELISA	1:500-1000