APG4B Polyclonal Antibody

$Formalin-fixed and paraffin embedded mouse liver labeled with Anti-APG4B\/AUTL1 Polyclonal Antibody, Unconjugated (bs-1384R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded mouse liver labeled with Anti-APG4B\/AUTL1 Polyclonal Antibody, Unconjugated (bs-1384R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Image kindly submitted by M. G. Connor as part of the free sample program. The bacteria Zprojection image figure legend: RAW264.7 Macrophage cells infected with GFP-bacteria (MOI3), stained with ATG4B primary antibody (1:1000) and visualized with Alexa Flour 595 (red). Nucleus is stained with DAPI (blue). Coverslips mounted with Prolong Gold Antifade with DAPI. Zprojections generated using FIJI. Scale bar is 5um. Images courtesy of M. G. Connor 2014

U937 lysates probed with Rabbit Anti-ATG4B Polyclonal Antibody, Unconjugated (bs-1384R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Overview
Catalog #	bs-1384R
Product Name	APG4B Polyclonal Antibody
Applications	ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ATG4B
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	23192
Swiss Prot	Q9Y4P1
Subcellular location	Cytoplasm
Synonyms	APG4B; AUTL1; Cysteine protease ATG4B; AUT-like 1 cysteine endopeptidase; Autophagin-1; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; hAPG4B; ATG4B; KIAA0943
Background	Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200