HNF1A Polyclonal Antibody

$Formalin-fixed and paraffin embedded human kidney labeled with Anti-HNF1\/TCF1\/HNF1A Polyclonal Antibody, Unconjugated (bs-1405R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded human kidney labeled with Anti-HNF1\/TCF1\/HNF1A Polyclonal Antibody, Unconjugated (bs-1405R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (HNF1A) polyclonal Antibody, Unconjugated (bs-1405R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (HNF1A) polyclonal Antibody, Unconjugated (bs-1405R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
IHC-P
IHC-F
IF
Flow-Cyt
ICC/IF

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Sheep
Pig
Chicken
Zebrafish

Overview
Catalog #	bs-1405R
Product Name	HNF1A Polyclonal Antibody
Applications	WB IHC-P IHC-F IF FLOW-CYT ICC/IF
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Sheep, Pig, Chicken, Zebrafish
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from rat HNF1
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	24817
Swiss Prot	P15257
Subcellular location	Nucleus
Synonyms	HNF1; Lfb1; Tcf1; LF-B1; Hepatocyte nuclear factor 1-alpha; HNF-1-alpha; HNF-1A; Liver-specific transcription factor LF-B1; Transcription factor 1; TCF-1; Hnf1a; Hnf-1
Background	Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
Application Dilution
WB	1:300-5000
IHC-P	ELISA1:500-1000
IHC-F	FCM1:20-100
IF	IHC-P1:200-400
Flow-Cyt
ICC/IF