TRIM14 Polyclonal Antibody

$Lane 1: Mouse Liver tissue lysates; Lane 2: Mouse Spleen tissue lysates probed with TRIM14 Polyclonal Antibody, Unconjugated (bs-16724R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Liver tissue lysates; Lane 2: Mouse Spleen tissue lysates probed with TRIM14 Polyclonal Antibody, Unconjugated (bs-16724R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (TRIM14) Polyclonal Antibody, Unconjugated (bs-16724R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (TRIM14) Polyclonal Antibody, Unconjugated (bs-16724R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

WB
IHC-P

Reactivity

Human
Mouse

Predicted Reactivity

Dog
Pig
Rabbit

Overview
Catalog #	bs-16724R
Product Name	TRIM14 Polyclonal Antibody
Applications	WB, IHC-P
Reactivity	Human, Mouse
Predicted Reactivity	Dog, Pig, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human TRIM14
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	9830
Swiss Prot	Q14142
Subcellular location	Cytoplasm
Synonyms	5830400N10Rik; KIAA0129; pub; TRI14_HUMAN; TRIM14; tripartite motif protein 14; tripartite motif protein TRIM14; tripartite motif-containing 14; Tripartite motif-containing protein 14.
Application Dilution
WB	=1:500-2000
IHC-P	IHC-P=1:100-500

Applications

Reactivity

Predicted Reactivity

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