HSPC111 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HSPC111 Polyclonal Antibody, Unconjugated (bs-18087R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HSPC111 Polyclonal Antibody, Unconjugated (bs-18087R) at 1:400 overnight at 4\u00b0C, DAB staining.

Applications

ELISA
IHC-P
IHC-F
IF

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Dog
Cow
Sheep
Pig
Horse
Rabbit

Overview
Catalog #	bs-18087R
Product Name	HSPC111 Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Dog, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human HSPC111
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	51491
Swiss Prot	Q9Y3C1
Synonyms	CGI 117; HBV pre S2 trans regulated protein 3; HBV pre-S2 trans-regulated protein 3; HSPC185; Hypothetical protein HSPC111; NOP16; NOP16 Gene; NOP16 nucleolar protein homolog; NOP16 nucleolar protein homolog (yeast); NOP16_HUMAN; Nucleolar protein 16; Nucleolar protein 16 homolog; Nucleolar protein 16 homolog (yeast); OTTHUMP00000223274; OTTHUMP00000223277.
Background	NOP16 is transcriptionally regulated by c-Myc (MYC; MIM 190080), upregulated in breast cancer, and overexpression is associated with poor patient survival (Butt et al., 2008).[supplied by OMIM, Jun 2009]
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF