NMES1 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NMES1 Polyclonal Antibody, Unconjugated (bs-19295R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NMES1 Polyclonal Antibody, Unconjugated (bs-19295R) at 1:400 overnight at 4\u00b0C, DAB staining.

Applications

IHC-P
IHC-F
IF
ICC

Reactivity

Predicted Reactivity

Human

Overview
Catalog #	bs-19295R
Product Name	NMES1 Polyclonal Antibody
Applications	IHC-P, IHC-F, IF, ICC
Reactivity	Rat
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human NMES1
Immunogen Range	31-83/83
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	84419
Swiss Prot	Q9C002
Synonyms	C15orf48; FLJ22645; FOAP-11; MGC32925; NMES1; NMES1_HUMAN; Normal mucosa of esophagus-specific gene 1 protein; Protein FOAP-11.
Background	This gene was first identified in a study of human esophageal squamous cell carcinoma tissues. Levels of both the message and protein are reduced in carcinoma samples. In adult human tissues, this gene is expressed in the the esophagus, stomach, small intestine, colon and placenta. Alternatively spliced transcript variants that encode the same protein have been identified. [provided by RefSeq, Jun 2012]
Application Dilution
IHC-P	1:200-400
IHC-F	1:100-500
IF
ICC	1:100-500

Applications

Reactivity

Predicted Reactivity

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