CYP2C9 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CYP2C9 Polyclonal Antibody, Unconjugated (bs-20243R) at 1:500 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CYP2C9 Polyclonal Antibody, Unconjugated (bs-20243R) at 1:500 overnight at 4\u00b0C, DAB staining.

$Lane 1: Mouse Liver tissue lysates; Lane 2: Rat Liver tissue lysates probed with CYP2C9 Polyclonal Antibody, Unconjugated (bs-20243R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Liver tissue lysates; Lane 2: Rat Liver tissue lysates probed with CYP2C9 Polyclonal Antibody, Unconjugated (bs-20243R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

ELISA
IHC-P
IHC-F
IF
ICC/IF

Reactivity

Mouse
Rat

Predicted Reactivity

Human

Overview
Catalog #	bs-20243r
Product Name	CYP2C9 Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF, ICC/IF
Reactivity	Mouse, Rat
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CYP2C9
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1559
Swiss Prot	P11712
Subcellular location	Cytoplasm
Synonyms	Cytochrome P450 2C9; The Cytochrome P450 (P450) superfamily of enzymes is one of three enzyme CPC9; CYP2C; CYP2C10; CYP2C9; Cytochrome p4502C9; P450 MP; P450 PB 1; P450 PB1; P450IIC9; P450MP; S mephenytoin 4 hydroxylase; CP2C9_HUMAN.
Application Dilution
ELISA	=1:5000-10000
IHC-P	IHC-P=1:100-500
IHC-F	IHC-F=1:100-500
IF	IF=1:100-500
ICC/IF	ICC/IF=1:100-500