DAK Polyclonal Antibody

$HeLa cell lysates probed with DAK Polyclonal Antibody, unconjugated (bs-20314R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

HeLa cell lysates probed with DAK Polyclonal Antibody, unconjugated (bs-20314R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Cow
Sheep
Pig
Horse
Rabbit

Overview
Catalog #	bs-20314R
Product Name	DAK Polyclonal Antibody
Applications	ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human DAK
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	26007
Swiss Prot	Q3LXA3
Subcellular location	Cytoplasm, Nucleus, Extracellular matrix
Synonyms	Triokinase/FMN cyclase; TKFC; Triokinase/FMN cyclase; Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing); ATP-dependent dihydroxyacetone kinase; DHA kinase; Glycerone kinase; Triokinase; Triose kinase; FAD-AMP lyase (cyclizing); FAD-AMP lyase (cyclic FMN forming); FMN cyclase
Background	Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate. Represses IFIH1-mediated cellular antiviral response.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200