MeCP2 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MeCP2 Polyclonal Antibody, Unconjugated (bs-22098R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MeCP2 Polyclonal Antibody, Unconjugated (bs-22098R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

IHC-P
IHC-F
IF

Reactivity

Predicted Reactivity

Human
Mouse
Dog
Cow
Sheep
Pig
Horse

Overview
Catalog #	bs-22098R
Product Name	MeCP2 Polyclonal Antibody
Applications	IHC-P, IHC-F, IF
Reactivity	Rat
Predicted Reactivity	Human, Mouse, Dog, Cow, Sheep, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human MeCP2
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4204
Swiss Prot	P51608
Subcellular location	Nucleus
Synonyms	AUTSX 3; AUTSX3; Mbd 5; MECP-2; MeCP 2 protein; Methyl CpG binding protein 2 (Rett syndrome); Methyl CpG binding protein 2; MRX 16; MRX 79; MRX16; MRX79; MRXS 13; MRXS13; MRXSL; PPMX; RTS; RTT; WBP 10; WBP10. MECP2_HUMAN
Background	KLH conjugated synthetic peptide derived from human MeCP2
Application Dilution
IHC-P	1:200-400
IHC-F
IF