RIM2 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RIM2 Polyclonal Antibody, Unconjugated (bs-22118R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RIM2 Polyclonal Antibody, Unconjugated (bs-22118R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

IHC-P
IHC-F
IF

Reactivity

Predicted Reactivity

Human
Mouse
Dog
Cow
Sheep
Pig
Horse
Chicken

Overview
Catalog #	bs-22118R
Product Name	RIM2 Polyclonal Antibody
Applications	IHC-P, IHC-F, IF
Reactivity	Rat
Predicted Reactivity	Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human RIM2
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	9699
Swiss Prot	Q9UQ26
Subcellular location	Cell membrane
Synonyms	Non small cell lung cancer RimL3a protein; Rab3 interacting molecule 2; RAB3IP3; Regulating synaptic membrane exocytosis protein 2; Rims2; RIMS2_HUMAN.
Background	RIM2 is a rab effector involved in exocytosis. It may act as scaffold protein. It is thought to be an effector protein for Rab3, binding to Rab3 on synaptic vesicles in a GTP dependent manner.
Application Dilution
IHC-P	1:200-400
IHC-F
IF