IL-1RA Polyclonal Antibody, PE Conjugated

$A431 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103 and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IL-1RA Polyclonal Antibody(bs-2216R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).$

A431 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103 and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IL-1RA Polyclonal Antibody(bs-2216R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Applications

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Sheep
Pig

Overview
Catalog #	bs-2216R-PE
Product Name	IL-1RA Polyclonal Antibody, PE Conjugated
Applications	WB, FCM
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Sheep, Pig
Specifications
Conjugation	PE
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from mouse IL-1RA
Immunogen Range	26-120/178
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID	16181
Swiss Prot	P25085
Subcellular location	Cytoplasm, Secreted
Synonyms	IL-1ra; F630041P17Rik; Interleukin-1 receptor antagonist protein; IL-1RN; IRAP; IL1 inhibitor; Il1rn
Background	Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity.
Application Dilution
WB	1:300-5000
FCM	1:20-100

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