prox1 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (prox1) Polyclonal Antibody, Unconjugated (bs-25103R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (prox1) Polyclonal Antibody, Unconjugated (bs-25103R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

IHC-P
IHC-F
IF

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Cow
Sheep
Pig
Horse
Chicken
Rabbit

Overview
Catalog #	bs-25103r
Product Name	prox1 Polyclonal Antibody
Applications	IHC-P, IHC-F, IF
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Cow, Sheep, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human prox1
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5629
Swiss Prot	Q92786
Subcellular location	Nucleus
Synonyms	Homeobox prospero like protein; Homeobox prospero like protein PROX1; Prospero related homeobox 1;prox-1; PROX 1.
Application Dilution
IHC-P	=1:100-500
IHC-F	IHC-F=1:400-800
IF	IF=1:100-500