PTPN11 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded (mouse ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (PTPN11) Polyclonal Antibody, Unconjugated (bs-25352R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (mouse ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (PTPN11) Polyclonal Antibody, Unconjugated (bs-25352R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

$HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PTPN11 Polyclonal Antibody(bs-25352R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).$

HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PTPN11 Polyclonal Antibody(bs-25352R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Applications

IHC-P

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Cow
Sheep
Pig
Horse
Chicken

Overview
Catalog #	bs-25352R
Product Name	PTPN11 Polyclonal Antibody
Applications	IHC-P
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Cow, Sheep, Pig, Horse, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PTPN11
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5781
Swiss Prot	Q06124
Subcellular location	Cytoplasm
Synonyms	SHP2; PTN11_HUMAN; BPTP 3; BPTP3; CFC; MGC14433; SHP 2; Noonan syndrome 1; Noonan syndrome 1 protein tyrosine phosphatase 2C; NS 1; NS1; Protein tyrosine phosphatase 2C; Protein Tyrosine Phosphatase Non receptor Type 11; PTP 1D; PTP 2C; PTP1D; PTP2C; PTPN 11; SAP2; SH PTP2; SH PTP3; SH2 domain containing protein tyrosine phosphatase 2; SHIP2; SHP 2; SHP-2; SHPTP 2; SHPTP2; SHPTP3; SIT protein precursor; Syp; Tyrosine protein phosphatase non receptor type 11; Src homology 2 (SH2) domain containing phosphotyrosinephosphatase 2.
Application Dilution
IHC-P	1:200-400

Applications

Reactivity

Predicted Reactivity

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