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Strep-Tag II Antibody, HRP Conjugated

Applications

  • WB
  • ELISA

Reactivity

  • Tags
  • Others
Overview
Catalog # bs-33016R-HRP
Product Name Strep-Tag II Antibody, HRP Conjugated
Applications WB, ELISA
Reactivity Tags, Others
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide of Strep-Tag
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Secreted
Synonyms Strep; Strep Tag; Strep.Tag; Strep-Tactin;
Background The Strep-tag system is a method which allows the purification and detection of proteins by affinity chromatography. The Strep-tag is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000