Phospho-RSK3 (Thr353 + Thr356) Polyclonal Antibody
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-3365R |
| Product Name | Phospho-RSK3 (Thr353 + Thr356) Polyclonal Antibody |
| Applications |
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Horse, Guinea Pig |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human RSK3 around the phosphorylation site of Thr353/356 |
| Modification Site | Thr353 + Thr356 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 6196 |
| Subcellular location | Nucleus |
| Synonyms | 90 kDa ribosomal protein S6 kinase 2; HU 2; KS6A2_HUMAN; MAP kinase activated protein kinase 1c; MAP kinase-activated protein kinase 1c; MAPK-activated protein kinase 1c; MAPKAP kinase 1c; MAPKAPK-1c; MAPKAPK1C; p90-RSK 2; p90RSK 2; p90RSK2; pp90RSK 3; pp90RSK3; Ribosomal protein S6 kinase alpha-2; Ribosomal S6 kinase 3; RPS6KA2; RSK 3; RSK-3; S6K alpha 2; S6K alpha; S6K-alpha-2. |
| Background | RSK3(Ribosomal S6 kinase 3)is a member of a family of 90-kDa ribosomal protein S6 kinases, which includes Rsk1, Rsk2 and Rsk3. These are broadly expressed serine/threonine protein kinases that are activated in response to mitogenic stimuli, including extracellular signal-regulated protein kinases Erk1 and Erk2. Rsk3 is a distinct isoform of p90Rsk that translocates to the cell nucleus, phosphorylates potential nuclear targets and may have a unique upstream activator. Several sites, such as Ser380, Thr359, Ser363 and Thr573, are important for its activation. Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c Fos and CREB. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |