PABP (Methyl Arg455/460) Antibody, Biotin Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Mouse
  • Rat
  • Others

Predicted Reactivity

  • Human
  • Cow
  • Pig
  • Horse
  • Chicken
  • Rabbit
  • Guinea Pig
Overview
Catalog # bs-3836R-Biotin
Product Name PABP (Methyl Arg455/460) Antibody, Biotin Conjugated
Applications WB, ELISA, IHC-P, IHC-F
Reactivity Mouse, Rat, Others
Predicted Reactivity Human, Cow, Pig, Horse, Chicken, Rabbit, Guinea Pig
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic metylpeptide derived from human PABP around metylation site of Arg455/Arg460
Modification Site Methyl Arg455/460
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 26986
Swiss Prot P11940
Subcellular location Cytoplasm, Nucleus
Synonyms PAB1; PABP; PABP1; PABPC2; PABPL1; Polyadenylate-binding protein 1; PABP-1; Poly(A)-binding protein 1; PABPC1
Background Binds the poly(A) tail of mRNA, including that of its own transcript. May be involved in cytoplasmic regulatory processes of mRNA metabolism such as pre-mRNA splicing. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500