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SPARC Polyclonal Antibody, Biotin Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
Overview
Catalog # bs-41345R-Biotin
Product Name SPARC Polyclonal Antibody, Biotin Conjugated
Applications WB, ELISA, IHC-P, IHC-F
Reactivity Mouse, Rat
Predicted Reactivity Human
Specifications
Conjugation Biotin
Host Rabbit
Source Recombinant SPARC protein
Clonality Polyclonal
Clone # #REF!
Isotype IgG
Concentration 1mg/ml
Purification affinity purified by Protein A
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C for 12 months.
Target
Gene ID 6678
Swiss Prot P09486
Subcellular location Extracellular, Secreted, Cell membrane
Synonyms AA517111; Basement membrane protein 40; BM 40; BM40; Cysteine rich protein; hm:zeh0062; MGC128090; ON; Osteonectin; Secreted acidic cystein rich glycoprotein; Secreted protein acidic and rich in cysteine; Secreted protein acidic cysteine rich (osteonectin); Secreted protein acidic cysteine rich; SPRC; SPRC_ .
Background Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
Application Dilution
WB =1:500-2000
ELISA ELISA=1:5000-10000
IHC-P IHC-P=1:400-800
IHC-F IHC-F=1:400-800