FCGR1A/CD64 Polyclonal Antibody

$The THP-1 (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-FCGR1A\/CD64 antibody (bs-41395R): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 \u03bcg\/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The THP-1 (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-FCGR1A\/CD64 antibody (bs-41395R): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 \u03bcg\/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

$Blank control\uff08black line\uff09:Raw264.7. \r\n \r\nPrimary Antibody (green line): Rabbit Anti-CD64 antibody (bs-41395R) \r\n\r\nDilution:1ug\/Test; \r\n \r\nSecondary Antibody\uff08white blue line\uff09: Goat anti-rabbit IgG-AF488\r\n\r\nDilution: 0.5ug\/Test. \r\n\r\nIsotype control\uff08orange line\uff09: Normal Rabbit IgG\r\n\r\nProtocol\r\n\r\nThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.$

Blank control\uff08black line\uff09:Raw264.7. \r\n \r\nPrimary Antibody (green line): Rabbit Anti-CD64 antibody (bs-41395R) \r\n\r\nDilution:1ug\/Test; \r\n \r\nSecondary Antibody\uff08white blue line\uff09: Goat anti-rabbit IgG-AF488\r\n\r\nDilution: 0.5ug\/Test. \r\n\r\nIsotype control\uff08orange line\uff09: Normal Rabbit IgG\r\n\r\nProtocol\r\n\r\nThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Applications

Flow-Cyt

Reactivity

Human
Mouse

Overview
Catalog #	bs-41395r
Product Name	FCGR1A/CD64 Polyclonal Antibody
Applications	Flow-Cyt
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant mouse FCGR1A (CD64) protein
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2209
Subcellular location	Cell membrane
Synonyms	High affinity immunoglobulin gamma Fc receptor I; CD 64; CD64 antigen; CD64A; Fc fragment of IgG high affinity Ia receptor; Fc fragment of IgG, high affinity Ia, receptor(CD64); Fc fragment of IgG, high affinity Ia, receptor for; Fc fragment of IgG, high affinity Ib, receptor(CD64); Fc fragment of IgG, high affinity Ib, receptor for; Fc fragment of IgG, high affinity Ic, receptor(CD64); Fc fragment of IgG, high affinity Ic, receptor for; Fc gamma receptor; Fc gamma receptor I A1; Fc gamma receptor I; Fc gamma receptor I B2; Fc gamma RI; Fc of IgG high affinity Ia receptor; Fc-gamma RIC; FCG 1; FCG1; FcgammaRI; FCGR 1; FCGR1; FCGR1A; FCGR1B; FCGR1C; FcRI; FCRIC; IGFR 1; IGFR1; IGFRB; IGFRC; IgG Fc receptor I; IgG Fc receptor IC; Immunoglobulin G Fc receptor I; Immunoglobulin G Fc receptor IB; Immunoglobulin G Fc receptor IC.
Application Dilution
Flow-Cyt	=1ug/Test