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DSN1 Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (DSN1) Polyclonal Antibody, Unconjugated (bs-42142R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (DSN1) Polyclonal Antibody, Unconjugated (bs-42142R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (DSN1) Polyclonal Antibody, Unconjugated (bs-42142R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (DSN1) Polyclonal Antibody, Unconjugated (bs-42142R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

  • ELISA
  • IHC-P

Reactivity

  • Human

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Horse
  • Rabbit
Overview
Catalog # bs-42142R
Product Name DSN1 Polyclonal Antibody
Applications ELISA, IHC-P
Reactivity Human
Predicted Reactivity Dog, Cow, Sheep, Horse, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant human DSN1 protein
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 79980
Swiss Prot Q9H410
Subcellular location Nucleus
Synonyms C20orf172; dJ469A13.2; DSN1; DSN1, MIND kinetochore complex component, homolog; DSN1, MIND kinetochore complex component, homolog (S. cerevisiae); DSN1_HUMAN; FLJ13346; Kinetochore associated protein DSN1; Kinetochore associated protein DSN1 homolog; Kinetochore-associated protein DSN1 homolog; MGC32987; MIS13.
Application Dilution
ELISA =1:5000-10000
IHC-P IHC-P=1:100-500

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