Collagen IV Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-Collagen IV Polyclonal Antibody, Unconjugated (bs-4595R) at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated (bs-0295G-PE) used at 1:200 dilution for 40 minutes at 37\u00b0C. DAPI was used to stain the cell nuclei$

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-Collagen IV Polyclonal Antibody, Unconjugated (bs-4595R) at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated (bs-0295G-PE) used at 1:200 dilution for 40 minutes at 37\u00b0C. DAPI was used to stain the cell nuclei

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human

Overview
Catalog #	bs-4595R
Product Name	Collagen IV Polyclonal Antibody
Applications	WB ELISA FCM IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Collagen alpha-1(IV) chain
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1282
Swiss Prot	P02462
Subcellular location	Secreted, Cell membrane, Extracellular matrix
Synonyms	ICH; HANAC; POREN1; arresten; Collagen alpha-1(IV) chain; COL4A1
Background	Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200