H3K9me3 Polyclonal Antibody

$Histone (acid) extracts from HeLa cells (HeLa HE, 20 \u03bcg) were analysed by Western blot using the H3K9me3 antibody (bs-53029R) diluted 1:750 in TBS-Tween containing 5% skimmed milk. The location of the protein of interest is indicated on the right.$

Histone (acid) extracts from HeLa cells (HeLa HE, 20 \u03bcg) were analysed by Western blot using the H3K9me3 antibody (bs-53029R) diluted 1:750 in TBS-Tween containing 5% skimmed milk. The location of the protein of interest is indicated on the right.

Applications

Reactivity

Human
Mouse

Overview
Catalog #	bs-53029R
Product Name	H3K9me3 Polyclonal Antibody
Applications	WB
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Polyclonal antibody raised in rabbit against the region of histone H3 containing the trimethylated lysine 9 (H3K9me3)
Clonality	Polyclonal
Clone #	#REF!
Isotype	Not Determined
Concentration	Not Determined
Purification	Whole antiserum from rabbit containing 0.05% azide
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	8350
Swiss Prot	P68431
Background	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Application Dilution
WB	1:300-5000