Ash2 Polyclonal Antibody

$NIH3T3 cells were stained with the Ash2 antibody (bs-53055R) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the Ash2 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two is shown on the right.$

NIH3T3 cells were stained with the Ash2 antibody (bs-53055R) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the Ash2 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two is shown on the right.

To determine the titer, an ELISA was performed using a serial dilution of the Ash2 antibody (bs-53055R). The wells were coated with the peptides used for immunization of the host rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:24,000.

Applications

WB
ELISA
IF

Reactivity

Mouse

Overview
Catalog #	bs-53055R
Product Name	Ash2 Polyclonal Antibody
Applications	WB, ELISA, IF
Reactivity	Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Polyclonal antibody against mouse Ash2, using 3 different KLH-conjugated synthetic peptides, 2 containing an amino acid sequence from the central and 1 containing C-terminal part of the protein
Clonality	Polyclonal
Clone #	#REF!
Isotype	Not Determined
Concentration	Not Determined
Purification	Whole antiserum from rabbit containing 0.05% azide
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	9070
Swiss Prot	Q9UBL3
Synonyms	ASH2L, ASH2L1, ASH2L2, Bre2, ASH2-like protein, Set1/Ash2 histone methyltransferase complex subunit ASH2
Background	Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis.
Application Dilution
WB	1:500-1000
ELISA	ELISA1:100-500
IF	IF1:100-200