MEF2C(Thr300) Polyclonal Antibody

Lane 1: Mouse Muscle lysates; Lane 2: Mouse Lymph node lysates probed with MEF2C(Thr300) Polyclonal Antibody, Unconjugated (bs-5483R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

$Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Muscle tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat Muscle tissue lysates; Lane 5: Rat Spleen tissue lysates probed with phospho-MEF2C (Thr300) Polyclonal Antibody, Unconjugated (bs-5483R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.$

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Muscle tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat Muscle tissue lysates; Lane 5: Rat Spleen tissue lysates probed with phospho-MEF2C (Thr300) Polyclonal Antibody, Unconjugated (bs-5483R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

Applications

WB
ELISA

Reactivity

Mouse

Predicted Reactivity

Human
Rat
Dog
Cow
Pig
Chicken
Rabbit

Overview
Catalog #	bs-5483r
Product Name	MEF2C(Thr300) Polyclonal Antibody
Applications	WB ELISA
Reactivity	Mouse
Predicted Reactivity	Human, Rat, Dog, Cow, Pig, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human MEF2C around the phosphorylation site of Thr300
Modification Site	Thr300
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4208
Subcellular location	Nucleus
Synonyms	MEF2Cphospho T300; MADS box transcription enhancer factor 2 polypeptide C myocyte enhancer factor 2C; Myocyte enhancer factor 2C; Myocyte specic enhancer factor 2C; Similar to MADS box transcription enhancer factor 2 polypeptide C; MEF2C_HUMAN.
Background	MEF2C is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. This protein controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. It may also be involved in neurogenesis and in the development of cortical architecture.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000