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CSPG4 Polyclonal Antibody

Lane 1: Hela cell lysates; Lane 2: CSPG4 knockout (KO) HeLa cell lysates probed with CSPG4 Polyclonal Antibody, Unconjugated (bs-55049R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: CSPG4 knockout (KO) HeLa cell lysates probed with CSPG4 Polyclonal Antibody, Unconjugated (bs-55049R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: A375 cell lysates; Lane 2: Hela cell lysates probed with CSPG4 Polyclonal Antibody, Unconjugated (bs-55049R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: A375 cell lysates; Lane 2: Hela cell lysates probed with CSPG4 Polyclonal Antibody, Unconjugated (bs-55049R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-55049R
Product Name CSPG4 Polyclonal Antibody
Applications
WB IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant fusion protein containing a sequence corresponding to amino acids 1950-2210 of human CSPG4 (NP_001888.2).
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C for 12 months.
Target
Gene ID 1464
Swiss Prot Q6UVK1
Subcellular location Cytoplasm, Cell membrane
Synonyms CSPG4;HMW-MAA;MCSP;MCSPG;MEL-CSPG;MSK16;NG2
Background A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
Application Dilution
WB 1:300-5000
IF

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