DNMT1 Polyclonal Antibody

Lane 1: 293T cell lysates; Lane 2: SW480 cell lysates probed with DNMT1 Polyclonal Antibody, Unconjugated (bs-55056R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: 293T cell lysates; Lane 2: DNMT1 knockout (KO) 293T cell lysates probed with DNMT1 Polyclonal Antibody, Unconjugated (bs-55056R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$293T cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (DNMT1) polyclonal Antibody, Unconjugated (bs-55056R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

293T cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (DNMT1) polyclonal Antibody, Unconjugated (bs-55056R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

$Lane 1: Human Jurkat cell lysates; Lane 2: Human Molt-4 cell lysates; Lane 3: Human K562 cell lysates; Lane 4: Human 293T cell lysates ; Lane 5: Human U251 cell lysates ; Lane 6: Human HL60 cell lysates probed with DNMT1 Polyclonal Antibody, Unconjugated (bs-55056R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human Jurkat cell lysates; Lane 2: Human Molt-4 cell lysates; Lane 3: Human K562 cell lysates; Lane 4: Human 293T cell lysates ; Lane 5: Human U251 cell lysates ; Lane 6: Human HL60 cell lysates probed with DNMT1 Polyclonal Antibody, Unconjugated (bs-55056R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Human 293T cell (Control) lysates; Lane 2: DNMT1 knockout (KO) 293T cell lysates probed with DNMT1 Polyclonal Antibody, Unconjugated (bs-55056R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human 293T cell (Control) lysates; Lane 2: DNMT1 knockout (KO) 293T cell lysates probed with DNMT1 Polyclonal Antibody, Unconjugated (bs-55056R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IF(ICC)
IP
IF

Reactivity

Human

Overview
Catalog #	bs-55056R
Product Name	DNMT1 Polyclonal Antibody
Applications	WB IF(ICC) IP IF
Specificity	KO-Validated
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human DNMT1 (NP_001370.1).
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1786
Swiss Prot	P26358
Subcellular location	Nucleus
Synonyms	ADCADN;AIM;CXXC9;DNMT;HSN1E;MCMT;m.HsaI;DNMT1
Background	This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200
IP	1-2ug
IF