GDI2 Polyclonal Antibody

Lane 1: 293T cell lysates; Lane 2: GDI2 knockout (KO) 293T cell lysates probed with GDI2 Polyclonal Antibody, Unconjugated (bs-55083R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: HT-29 cell lysates; Lane 2: A549 cell lysates; Lane 3: HepG2 cell lysates; Lane 4: U87MG cell lysates; Lane 5: Mouse spleen lysates; Lane 6: Mouse kidney lysates; Lane 7: Mouse lung lysates; Lane 8: Rat liver lysates; Lane 9: Rat spinal cord lysates probed with GDI2 Polyclonal Antibody, Unconjugated (bs-55083R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$NIH\/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (GDI2) polyclonal Antibody, Unconjugated (bs-55083R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

NIH\/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (GDI2) polyclonal Antibody, Unconjugated (bs-55083R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
IF(ICC)
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-55083R
Product Name	GDI2 Polyclonal Antibody
Applications	WB, IF(ICC), IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 296-445 of human GDI2 (NP_001485.2).
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	2665
Swiss Prot	P50395
Subcellular location	Cytoplasm, Cell membrane
Synonyms	GDI2;HEL-S-46e;RABGDIB
Background	GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI2 is ubiquitously expressed. The GDI2 gene contains many repetitive elements indicating that it may be prone to inversion/deletion rearrangements. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200
IF

Applications

Reactivity

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