HPRT1 Polyclonal Antibody

Lane 1: 293T cell lysates; Lane 2: HPRT1 knockout (KO) 293T cell lysates probed with HPRT1 Polyclonal Antibody, Unconjugated (bs-55102R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: MCF-7 lysates; Lane 2: HL-60 cell lysates; Lane 3: SW480 cell lysates; Lane 4: 293T cell lysates; Lane 5: Hela cell lysates; Lane 6: Mouse brain lysates; Lane 7: Mouse Liver lysates probed with HPRT1 Polyclonal Antibody, Unconjugated (bs-55102R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$U2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (HPRT1 ) polyclonal Antibody, Unconjugated (bs-55102R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

U2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (HPRT1 ) polyclonal Antibody, Unconjugated (bs-55102R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
IF(ICC)
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-55102R
Product Name	HPRT1 Polyclonal Antibody
Applications	WB, IF(ICC), IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 1-218 of human HPRT1 (NP_000185.1).
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	3251
Swiss Prot	P00492
Subcellular location	Cytoplasm
Synonyms	HPRT1;HGPRT;HPRT
Background	The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200
IF