NDUFA7 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human liver damage; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NDUFA7 Polyclonal Antibody, Unconjugated (bs-55144R) at 1:100 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human liver damage; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NDUFA7 Polyclonal Antibody, Unconjugated (bs-55144R) at 1:100 overnight at 4\u00b0C, DAB staining.

Lane 1: 293T cell lysates; Lane 2: NDUFA7 knockout (KO) 293T cell lysates probed with NDUFA7 Polyclonal Antibody, Unconjugated (bs-55144R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: A549 cell lysates; Lane 2: HepG2 cell lysates; Lane 3: MCF-7 cell lysates; Lane 4: HL-60 cell lysates; Lane 5: Mouse Kidney lysates; Lane 6: Mouse Brain lysates; Lane 7: Mouse Liver lysates; Lane 8: Mouse Heart lysates; Lane 9: Rat Liver lysates probed with NDUFA7 Polyclonal Antibody, Unconjugated (bs-55144R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-55144R
Product Name	NDUFA7 Polyclonal Antibody
Applications	WB, IHC-P, IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 1-113 of human NDUFA7 (NP_004992.2).
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	4701
Swiss Prot	O95182
Subcellular location	Cytoplasm
Synonyms	NDUFA7;B14.5a;CI-B14.5a
Background	This gene encodes a subunit of NADH:ubiquinone oxidoreductase (complex I), which is a multiprotein complex located in the inner mitochondrial membrane. Complex I functions in the transfer of electrons from NADH to the respiratory chain.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC