PDCD10 Polyclonal Antibody

$Lane 1: Hela cell lysates; Lane 2: PDCD10 knockout (KO) HeLa cell lysates probed with PDCD10 Polyclonal Antibody, Unconjugated (bs-55165R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Hela cell lysates; Lane 2: PDCD10 knockout (KO) HeLa cell lysates probed with PDCD10 Polyclonal Antibody, Unconjugated (bs-55165R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

$Lane 1: A431 cell lysates; Lane 2: HepG2 cell lysates; Lane 3: Jurkat cell lysates; Lane 4: MCF-7 cell lysates; Lane 5: Mouse Thymus lysates; Lane 6: Mouse Brain lysates probed with PDCD10 Polyclonal Antibody, Unconjugated (bs-55165R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: A431 cell lysates; Lane 2: HepG2 cell lysates; Lane 3: Jurkat cell lysates; Lane 4: MCF-7 cell lysates; Lane 5: Mouse Thymus lysates; Lane 6: Mouse Brain lysates probed with PDCD10 Polyclonal Antibody, Unconjugated (bs-55165R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

Reactivity

Human
Mouse

Overview
Catalog #	bs-55165R
Product Name	PDCD10 Polyclonal Antibody
Applications	WB IF
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 1-212 of human PDCD10 (NP_009148.2).
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	11235
Swiss Prot	Q9BUL8
Subcellular location	Cytoplasm, Cell membrane
Synonyms	PDCD10;CCM3;TFAR15
Background	This gene encodes an evolutionarily conserved protein associated with cell apoptosis. The protein interacts with the serine/threonine protein kinase MST4 to modulate the extracellular signal-regulated kinase (ERK) pathway. It also interacts with and is phosphoryated by serine/threonine kinase 25, and is thought to function in a signaling pathway essential for vascular developent. Mutations in this gene are one cause of cerebral cavernous malformations, which are vascular malformations that cause seizures and cerebral hemorrhages. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Application Dilution
WB	1:300-5000
IF