SAMHD1 Polyclonal Antibody

Lane 1: Hela cell lysates; Lane 2: SAMHD1 knockout (KO) HeLa cell lysates probed with SAMHD1 Polyclonal Antibody, Unconjugated (bs-55191R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: HepG2 cell lysates; Lane 2: Hela cell lysates; Lane 3: MCF-7 cell lysates; Lane 4: Mouse Spleen lysates probed with SAMHD1 Polyclonal Antibody, Unconjugated (bs-55191R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (SAMHD1) polyclonal Antibody, Unconjugated (bs-55191R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (SAMHD1) polyclonal Antibody, Unconjugated (bs-55191R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
IF(ICC)
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-55191R
Product Name	SAMHD1 Polyclonal Antibody
Applications	WB, IF(ICC), IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 387-626 of human SAMHD1 (NP_056289.2).
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	25939
Swiss Prot	Q9Y3Z3
Subcellular location	Nucleus
Synonyms	SAMHD1;CHBL2;DCIP;HDDC1;MOP-5;SBBI88
Background	This gene may play a role in regulation of the innate immune response. The encoded protein is upregulated in response to viral infection and may be involved in mediation of tumor necrosis factor-alpha proinflammatory responses. Mutations in this gene have been associated with Aicardi-Goutieres syndrome.
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200
IF