UBE2C Polyclonal Antibody

$Lane 1: Hela cell lysates; Lane 2: UBE2C knockout (KO) HeLa cell lysates probed with UBE2C Polyclonal Antibody, Unconjugated (bs-55218R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Hela cell lysates; Lane 2: UBE2C knockout (KO) HeLa cell lysates probed with UBE2C Polyclonal Antibody, Unconjugated (bs-55218R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

Reactivity

Human

Overview
Catalog #	bs-55218R
Product Name	UBE2C Polyclonal Antibody
Applications	WB IP IHC IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human UBE2C (NP_008950.1).
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	11065
Swiss Prot	O00762
Subcellular location	Cytoplasm, Nucleus, Cell membrane
Synonyms	UBE2C;UBCH10;dJ447F3.2
Background	The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is required for the destruction of mitotic cyclins and for cell cycle progression, and may be involved in cancer progression. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been defined on chromosomes 4, 14, 15, 18, and 19.
Application Dilution
WB	1:300-5000
IP	1-2ug
IHC
IF