Butyryl-Histone H2B (Lys15) Polyclonal Antibody

$Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 14 kDa\r\nObserved MW: 14 kDa$

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 14 kDa\r\nObserved MW: 14 kDa

Applications

Reactivity

Human
Mouse

Overview
Catalog #	bs-60190R
Product Name	Butyryl-Histone H2B (Lys15) Polyclonal Antibody
Applications	WB, ICC
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Protein A coupled with the antigen pepetide containing butyrylated H2B (Lys15).
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot	P62807
Application Dilution
WB	1:300-5000
ICC	1:100-500

Applications

Reactivity

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