KIST Polyclonal Antibody

$Lane 1: rat brain lysates Lane 2: human colon carcinoma lysates probed with Anti KIST\/UHMK1 Polyclonal Antibody, Unconjugated (bs-6260R) at 1:200 in 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 47kD. Observed band size: 47kD.$

Lane 1: rat brain lysates Lane 2: human colon carcinoma lysates probed with Anti KIST\/UHMK1 Polyclonal Antibody, Unconjugated (bs-6260R) at 1:200 in 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 47kD. Observed band size: 47kD.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Predicted Reactivity

Human
Mouse
Rat
Cow
Pig
Rabbit

Overview
Catalog #	bs-6260R
Product Name	KIST Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity	Human, Mouse, Rat, Cow, Pig, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human KIST/UHMK1
Immunogen Range	331-419/419
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	127933
Swiss Prot	Q8TAS1
Subcellular location	Nucleus
Synonyms	KIS; KIST; P-CIP2; Serine/threonine-protein kinase Kist; Kinase interacting with stathmin; PAM COOH-terminal interactor protein 2; U2AF homology motif kinase 1; UHMK1
Background	Upon serum stimulation, phosphorylates CDKN1B/p27Kip1, thus controlling CDKN1B subcellular location and cell cycle progression in G1 phase. May be involved in trafficking and/or processing of RNA (By similarity).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200